Tuesday, 28 October 2003
D0272

This presentation is part of : Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Detection of knockdown resistance in permethrin-resistant populations of the human head louse, Pediculus capitis, in the USA

Jian-Rong Gao1, Kyong Sup Yoon1, Si Hyeock Lee2, Miwako Takano-Lee3, John D. Edman3, Terri L. Meinking4, David Taplin4, and J. Marshall Clark1. (1) University of Massachusetts, Department of Entomology, Fernald Hall, Amherst, MA, (2) Seoul National University, School of Agricultural Biotechnology, College of Agriculture and Life Science, Suwon, South Korea, (3) University of California, Department of Entomology, Davis, CA, (4) University of Miami, Department of Dermatology and Cutaneous Surgery, School of Medicine, Miami, FL

Head louse populations from California, Florida and Texas showed (1)1.5-, 3.1- and 1.5- to 5.1-fold of permethrin resistance, respectively, compared to permethrin-susceptible head louse populations from Panama or Ecuador, using insecticide impregnated, filter disk-contact bioassay, (2) 0.53, 0.97 and 0.33 to 1.00 of resistant allele frequency, respectively, by DNA sequence analysis. The resistance was determined to be complete recessive and highly correlated with the presence of the T929I and L932F point mutations, which are suitable for detection by a variety of DNA-based diagnostic techniques.

Species 1: Phthiraptera Pediculidae Pediculus capitis (human head lice)
Keywords: permethrin, resistance

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