Wednesday, 29 October 2003 - 8:00 AM
0853

This presentation is part of : Ten-Minute Papers, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Stage- and tissue-specific regulation of allatotropin gene expression

Frank M. Horodyski1, Kyeong-Yeoll Lee2, Mary E. Chamberlin3, and Michelle M. Elekonich1. (1) Ohio University, Biomedical Sciences, 228 Irvine Hall, Athens, OH, (2) Kyungpook National University, Department of Agricultural Biology, Daegu, South Korea, (3) Ohio University, Biological Sciences, 108 Irvine Hall, Athens, OH

Allatotropin is a member of a family of myotropic neuropeptides that stimulate juvenile hormone biosynthesis in some insects including the adult Lepidoptera. In addition, Manduca sexta allatotropin (Manse-AT) inhibits active ion transport across the larval midgut epithelium. The Manse-AT gene is expressed as at least three mRNAs that differ by alternative splicing. Each mRNA is predicted to encode a precursor protein from which Manse-AT is derived. Two Manse-AT mRNAs contain additional exons that contain the coding sequence of peptides related to Manse-AT; allatotropin-like (ATL) peptides. The ATL peptide possess similar biological activities as Manse-AT on the adult corpora allata and the larval midgut. The Manse-AT gene is expressed in multiple regions of the larval and adult central and enteric nervous systems consistent with its multiple biological roles. Alternative splicing of the Manse-AT gene is regulated in a stage- and tissue-specific manner, and the levels of one Manse-AT mRNA are specifically increased in response to nutrient deprivation. This work was supported by NSF.

Species 1: Lepidoptera Sphingidae Manduca sexta (tobacco hornworm)
Keywords: neuropeptide, juvenile hormone

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