Monday, 27 October 2003
D0031

This presentation is part of : Student Competition Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Expression and characterization studies of Microplitis demolitor bracovirus

Honglada Thoetkiattikul1, Markus H. Beck1, Mark D. Lavine1, Stephanie Deboor2, Bruce A. Webb2, and Michael R. Strand1. (1) University of Georgia, Entomology, 413 Biological Science Building, Athens, GA, (2) University of Kentucky, Entomology, S-225 Agriculture Science Center North, Lexington, KY

Microplitis demolitor bracovirus (MdBV) is specifically associated with the braconid wasp Microplitis demolitor which parasitizes the larval stage of the moth Pseudoplusia includens (Lepidoptera: Noctuidae). The MdBV genome consists of approximately 15 non-equimolar, circular and segmented DNAs. Among these DNAs, we have identified four gene families: the EGF genes, Glc-genes, Ank-repeat genes, and PTP genes. In this we characterized temporal-spatial pattern of expression of these gene families in parasitized hosts by real-time PCR.

Species 1: Hymenoptera Braconidae Microplitis demolitor
Species 2: Lepidoptera Noctuidae Pseudoplusia includens
Keywords: polydnavirus

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