Tuesday, 28 October 2003
D0375

This presentation is part of : Display Presentations, Section D. Medical and Veterinary Entomology

Modified K & D module for in vitro screening of candidate mosquito repellents

Jerry Klun1, Matthew Kramer1, and Mustapha Debboun2. (1) USDA Agricultural Research Service, Beltsville Agricultural Research Center, 10300 Baltimore Ave, Beltsville, MD, (2) Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD

A modified K & D module was designed and protocols for use of the module were developed to quantify mosquito biting responses in vitro. Dose-response assays were conducted against Aedes aegypti using SS220, Deet and Bayrepel, and a fixed dose of each repellent was tested against Anopheles stephensi and A. aegypti. Estimated dose response curves and estimated proportions of repelled mosquitoes at the fixed dose were compared to data estimates obtained earlier using in vivo human volunteer assay. Data show that the in vitro assay results correlate with in vivo assays.

Species 1: Diptera Culicidae Aedes aegypti (yellow fever mosquito)
Species 2: Diptera Culicidae Anopheles stephensi
Keywords: SS220, Deet

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