Sunday, 26 October 2003 - 3:36 PM
0156

This presentation is part of : Ten-Minute Papers, Section Cd. Behavior and Ecology

Identification of sexual pheromone of Copitarsia incommoda (Lepidoptera: Noctuidae)

Juan Cibrián-Tovar1, Julio Rojas2, Sean Putnam3, Gloria Calyecac1, Leopoldo Cruz-Lopez2, Edi Malo2, and Ovidio Diaz-Gomez4. (1) Colegio de Postgraduados, IFIT, Km36.5 carretera Mexico-Texcoco, Montecillo, Edo. de Mexico, Mexico, (2) El Colegio de la Frontera Sur, Departamento de Entomologia Tropical, Apdo. Postal 36, Tapachula, Chiapas, Mexico, (3) University of Nebraska-Lincoln, Entomology Department, 311 Plant Industry Bldg, Lincoln, NE, (4) Universidad de San Luis Potosi, Facultad de Agronomia, Alvaro Obregon 64, San Luis Potosi, San Luis Potosi, Mexico

Abstract- Chemical, electrophysiological analysis and field trials were used to identify the female sex pheromone of Copitarsia incommoda (=C. consueta)(Lepidoptera: Noctuidae), an important pest of several crops in Latin America. Coupled gas chromatographic-electroantennographic detection (GC-EAD) analysis showed the presence of two EAD- active compounds produced by females. The compounds were identified as Z9-tetradecenyl acetate (Z9-14: Ac) and Z9-tetradecenol (Z9-14:OH) by GC-EAD and comparative GC-mass spectrometric (GC-MS) analysis of the female-produced candidate pheromone and synthetic standards. In addition to Z9-14: Ac and Z9-14: OH, GC-MS analysis of abdomen washing and entrained volatiles showed the presence of three additional compounds. These compounds were identified as dodecyl acetate (12:Ac), tetradecyl acetate (14: Ac) and (Z,Z,Z)-3,6,9-tricosatriene. The electroantennogram (EAG) evaluation of the all five compounds at different doses, showed that 14: Ac and (Z,Z,Z)-3,6,9-tricosatriene were electrophysiologically inactive. Field trials with traps baited with single, binary and tertiary blends of 12:Ac, Z9-14: Ac and Z9-14: OH, showed that none of the three components were attractive to males when tested as singled components. However, the combination of Z9-14: ac and Z9-14: OH was critical for female attraction.



Species 1: Lepidoptera Noctuidae Copitarsia incommoda
Keywords: Chemical identification

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