To understand molecular basis involved in insecticide resistance development, we have attempted to use a combination of subtractive hybridization and complementary DNA (cDNA) array techniques to monitor genes differentially expressed (constitutive and permethrin induced) within the entire genome of house flies response to insecticides. We have constructed resistant-susceptible and permethrin treated-untreated house fly subtractive libraries. By hybridizing cDNA arrays of PCR amplified cDNAs of over 3000 clones from each subtractive library with 32P labeled cDNA probes of resistant, susceptible, and permethrin treated house flies, we obtained 6 genes that are specifically expressed in a resistant strain compared to a susceptible strain and 7 genes that are differentially expressed between permethrin treated and untreated house flies. The functions of these genes and their possible roles in response to insecticide resistance will be discussed.
Species 1: Diptera Muscidae Musca domestica (House fly)
Keywords: resistance
Back to Ten-Minute Papers, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology
Back to Ten-Minute Papers, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology
Back to The 2002 ESA Annual Meeting and Exhibition