Monday, 18 November 2002
D0156

This presentation is part of : Display Presentations, Section A. Systematics, Morphology, and Evolution

Attempts to introduce Wolbachia into uninfected parasitoids via rearing on a Wolbachia-infected host: False positives and ultimate failure

George K. Kyei-Poku, Kevin D. Floate, Bernhard Benkel, and Mark S. Goettel. Lethbridge Research Centre, Agriculture & Agri-Food Canada, P.O. Box 3000, Lethbridge, AB, Canada

Wolbachia are bacteria that infect more than 20 percent of arthropod species. Infections have profound implications for host reproduction, including cytoplasmic incompatibility, parthenogenesis, male-killing, and feminization. Transfer of Wolbachia between species has been hypothesized between parasitic wasps and their dipteran hosts. We attempted to transfer Wolbachia between species by rearing Wolbachia-free, Muscidifurax raptor, M. zaraptor and M. raptorellus (Hymenoptera: Pteromalidae) on Wolbachia-infected horn fly, Haematobia irritans (Diptera: Muscidae) for 10 consecutive generations. Wolbachia was detected in M. raptor in Generation 7, but no other positive results were recorded.

Species 1: Hymenoptera Pteromalidae Muscidifurax raptor
Species 2: Hymenoptera Pteromalidae Muscidifurax zaraptor
Species 3: Diptera Muscidae Haematobia irritans (horn fly)
Keywords: Wolbachia, horizontal transmission

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