Wednesday, 20 November 2002
D0502

This presentation is part of : Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Identification of 20E response proteins in the salivary glands of the ecdysone-deficient woc^rgl mutant of Drosophila melanogaster using proteomic approaches

Differential display technique was used to investigate the protein expression patterns in the salivary glands from the third instar larvae of both the wildtype and the ecdysone-deficient mutant without children^rgl (woc^rgl) of Drosophila melanogaster. Several woc mutant specific, ecdysone response proteins were isolated from two-dimensional (2-D) gel maps, trypsinated and subjected to MALDI-TOF analysis. One of these proteins was identified as a-tubulin by searching SWISS-PROT database and verified by Western blot analysis and immunocytochemistry using a-tubulin specific antibody. The expression of a-tubulin, not the b-tubulin was inhibited by 20E in woc mutant.

Species 1: Diptera Drosophilidae Drosophila melanogaster (pomace fly)
Keywords: alph tubulin, immunocytochemistry

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