Sunday, 17 November 2002 - 10:24 AM
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This presentation is part of : Ten-Minute Papers, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Molecular detection of Bt resistance in the field

Shai Morin1, Mark Sisterson2, Robert Biggs2, Judith K. Brown1, Timothy Dennehy2, Yves Carriere2, David G. Heckel3, and Bruce E. Tabashnik2. (1) University of Arizona, Plant Sciences, College of Agriculture, Forbes Building, Tucson, AZ, (2) University of Arizona, Department of Entomology, Forbes 410, Tucson, AZ, (3) University of Melbourne, Department of Genetics, Gate 12, Royal Parade, Parkville, Australia

Transgenic crops expressing insecticidal toxins from Bacillus thuringiensis (Bt) are widely used for pest control. Although laboratory selection results show that many pests have the genetic potential to evolve resistance to Bt toxins, the molecular basis of resistance had remained elusive for many years. It was shown previously that resistance to Cry1Ac in the cotton pest Heliothis virescens is associated with disruption of a cadherin-superfamily gene. We used polymorphic intron DNA markers to show tight linkage between a cadherin gene and resistance to Cry1Ac in a field-derived strain (AZP-R) of pink bollworm (Pectinophora gossypiella). We will discuss the benefits and drawbacks of using polymorphic intron DNA markers in monitoring Bt resistance in the field.

Species 1: Lepidoptera Gelechiidae Pectinophora Gossypiella (Pink Bollworm)
Keywords: Bt, Resistance

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