Monday, 18 November 2002
D0195

This presentation is part of : Display Presentations, Subsection Cc. Insect Vectors in Relation to Plant Disease

Polymerase chain reaction-restriction fragment length polymorphism as a diagnostic tool to distinguish between Frankliniella species (Thysanoptera: Thripidae)

Susan E. Webb, University of Florida, Entomology and Nematology Department, P.O. Box 110620. Natural Area Drive, Gainesville, FL and Heather L Smith, University of Florida, Department of Entomology and Nematology, PO Box 110620, Gainesville, FL.

Tomato spotted wilt virus tospovirus (TSWV) is spread exclusively by thrips and has a very broad host range that includes important economic crops such as tomatoes, peppers, and peanuts. It is vital to determine host plants that support both TSWV and thrips reproduction, and for this to occur, it is necessary to identify closely related species of thrips that are nearly indistinguishable morphologically. For this study, a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) procedure was developed to distinguish between Frankliniella bispinosa, F. fusca, and F. occidentalis.



Species 1: Thysanoptera Thripidae Frankliniella bispinosa
Species 2: Thysanoptera Thripidae Frankliniella fusca
Species 3: Thysanoptera Thripidae Frankliniella occidentalis
Keywords: tomato spotted wilt virus, vector

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