Diapause is an optional, inducible developmental state in the flesh fly, Sarcophaga crassipalpis. We are interested in the molecular controls underlying the developmental process of the initation and maintenance of this state. A cDNA library was constructed from the brains of diapausing pupae of S. crassipalpis. Initial cDNA were constructed using RT-PCR and the cDNA inserts were cloned into bacteriophage vectors. Plasmids were rescued from the phage vector and initial characterization of the inserts has begun. We are using a Drosophila microarray to screen for diapause and nondiapause regulated genes an an attempt to assemble a set of representative genes for use in further analysis of this unique developmental state.
Species 1: Diptera Sarcophagidae Sarcophaga crassipalpis (flesh fly)
Keywords: differential-gene expression
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