The use of embryo cryopreservation of tephritid flies can be an important means for preserving the numerous strains used in genetic research. For this technology to be utilized as an economical and reliable means to indefinitely store fruit fly strains, it is imperative that the germplasm of the preserved insects remain unaltered. We have used RAPD analysis of the genomic DNA of Mexican fruit flies to examine indicators of genetic variation present before and after the cryopreservation treatment. Our data indicate that selection for a genotype most able to survive the stresses of cryopreservation does not occur.
Species 1: Diptera Tephritidae Anastrepha ludens (Mexican fruit fly)
Keywords: cold storage, RAPD analysis
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