Tuesday, 19 November 2002
D0289

This presentation is part of : Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Assessing the reproductive fitness of a mass-reared predator, Orius insidiosus

Jeffrey P. Shapiro and Stephen M. Ferkovich. USDA-ARS, Center for Medical, Agricultural, and Veterinary Entomology, 1600/1700 SW 23rd Drive, Gainesville, FL

Insectaries that rear beneficial arthropods assess the quality of their insects and shipments through a variety of procedures, such as manual counts of insects or oviposited eggs, or behavioral tests of mobility or reproductive vigor. Fecundity is a key measure of quality in adult mass-reared arthropods, and is normally determined by counting eggs throughout the life of a female insect. Since egg counts are at least laborious and often impractical, we have proposed a quantitative test of yolk protein content, the Yolk Protein Enzyme Linked Immunosorbent Assay (YP-ELISA). We can gain significant advantage in developing the YP-ELISA by understanding the dynamics of egg development and biochemical characteristics of the yolk proteins. The YP-ELISA itself lends quantitative insight into processes that are now primarily understood qualitatively.



Species 1: Heteroptera Anthocoridae Orius insidiosus (minute pirate bug, insidious flower bug)
Keywords: immunoassay, nutrition

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