Tuesday, 19 November 2002
D0275

This presentation is part of : Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Characterization of the polydnavirus associated with the parasitoid wasp Glyptapanteles indiensis

Yanping Chen and Dawn Gundersen-Rindal. IBL PSI ARS USDA, Bldg. 011A, Rm. 214, BARC West, Beltsville, MD

Glyptapanteles indiensis polydnavirus (GiPDV) is essential for successful parasitization of the lepidopteran Lymantria dispar by the endoparasitic wasp G. indiensis. This virus has not been characterized. Ultrastructural studies of GiPDV showed that the virions were ovoid and contained as many as ten nucleocapsids each enclosed by a single unit membrane envelope. Field Inversion Gel Electrophoresis (FIGE) analysis revealed that GiPDV had a segmented genome composed of 13 dsDNA segments, ranging in size from approximately 11 kb to more than 30 kb. Four genomic segments were presented in higher molar concentration than the others. Further characterization of the GiPDV genome indicated several GiPDV-specific mRNAs that were expressed in the parasitized host were present on more than one genomic DNA segment, suggesting the existence of homologous sequences among DNA segments, or nested genomic segments.

Species 1: Hymenoptera Braconidae Glyptapantales indiensis
Species 2: Lepidoptera Lymantriidae Lymantria dispar (gypsy moth)
Keywords: polydnavirus

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