Tuesday, 19 November 2002
D0266

This presentation is part of : Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Differential expression and induction of two Drosophila cytochrome P450 genes near the Rst(2)DDT locus

Amanda Brandt1, Michael E. Scharf1, Joao H. Pedra1, Gibran Holmes1, Andrea Dean1, Martin Kreitman2, and Barry R. Pittendrigh1. (1) Purdue University, Department of Entomology, 1158 Smith Hall, West Lafayette, IN, (2) University of Chicago, Department of Ecology and Evolution, 5801 South Ellis, Chicago, IL

Previous studies have shown that the major metabolic resistance locus in the insecticide-resistant Drosophila line Rst(2)DDTWisconsin maps between the markers cn and vg on chromosome 2. Six cytochrome P450 genes exist in this region. We investigated the expression levels of these P450 genes in DDT-resistant and -susceptible fly lines. We report: (i) DDT resistance is significant (> 30-fold) and dominant, (ii) resistance is reduced by the cytochrome P450 inhibitor PBO, (iii) there is constitutive over-expression relative to susceptible flies of two genes encoding cytochrome P450 enzymes within the cn-vg region (CYP6G1=4.3-fold; CYP12D1=6.0-fold), and (iv) exposure to DDT results in an increased expression of only one of these two P450 genes (CYP12D1 > 6-fold above constitutive resistant fly baselines).

Species 1: Diptera Drosophilidae Drosophila melanogaster
Keywords: resistance, insecticides

Back to Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology
Back to Posters
Back to The 2002 ESA Annual Meeting and Exhibition