Certain Mexican strains of Boophilus microplus (Corrales, San Felipe) with extremely high resistance to pyrethroid acaricides have a single point mutation in their sodium channel gene. Another Mexican strain (Coatzacoalcos) has a moderate level of resistance to pyrethroids and a point mutation in an esterase gene has been found in this strain. Methods that have been developed for these mutations based on PCR and agarose gel electrophoresis are relatively simple, but they require additional time and efforts after PCR. We are now developing fluorescence-based probes that would eliminate the need for post-PCR operations. The instrument now available can discern multiple fluorescent probes. When the wild-type and mutant probes are labeled with different fluorescent reporters, both alleles can be detected simultaneously.
Species 1: Acari Ixodidae Boophilus microplus (southern cattle tick)
Keywords: PCR, resistance
Back to Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology
Back to Posters
Back to The 2002 ESA Annual Meeting and Exhibition