Tuesday, 19 November 2002
D0273

This presentation is part of : Display Presentations, Section B. Physiology, Biochemistry, Toxicology, and Molecular Biology

Hagenís glands of the parasitic wasp Diachasmimorpha longicaudata (Hymenoptera: Braconidae): Ultrastructure and the detection of entomopoxvirus and parasitism-specific proteins

Cynthia C. Khoo and Pauline O. Lawrence. University of Florida, Department of Entomology and Nematology, P.O. Box 110620, 970 Natural Area Drive, Gainesville, FL

Hagenís glands of males of the parasitic wasp Diachasmimorpha longicaudata (Hymenoptera: Braconidae) secrete compounds that are involved in courtship and defense. Like the poison glands of female wasps, the Hagenís glands are secretory, membranous, and of ectodermal origin. The poison glands contain the symbiotic entomopoxvirus, DlEPV and the parasitism-specific protein, PSP 24. DlEPV proteins were detected in homogenates of male wasps. Our goal was to describe the ultrastructure of the Hagenís glands and determine whether they contain DlEPV virions and/or proteins as well as PSP 24. The Hagenís glands are bilateral and each consists of 12Ė16 tubules arranged in two clusters. In cross-section, a tubule has three zones that enclose a central cuticle-lined lumen. The outermost zone consists of aggregates (Ďislandsí) of small vesicles, interconnected by narrow ductules that lead to large cuticle-lined ducts, which transport electron-dense material to the lumen prior to its release from the gland. Large vesicles in Zone 2 and a thick layer of ribosomes and rough endoplasmic reticula in Zone 3 are the likely sites of storage and protein synthesis, respectively. While DlEPV virions were not seen in the Hagenís gland, DlEPV and PSP 24 proteins were present.

Species 1: Hymenoptera Braconidae Diachasmimorpha longicaudata
Species 2: Diptera Tephritidae Anastrepha suspensa (Caribbean fruit fly)
Keywords: poxvirus, parasitism-specific glycoprotein

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