Insecticide resistance to three organophosphate (chlorpyrifos-methyl, malathion and pirimiphos-methyl) and three pyrethroid (cypermethrin, deltamethrin and permethrin) insecticides was surveyed in nine field-collected populations of the maize weevil from six Brazilian States and contrasted with a standard susceptible population and a DDT and pyrethroid resistant population. Bioassay tests were carried out in glass-vials (20 ml) impregnated with dried insecticide residue and the resistant populations were recognised by using discriminating concentrations established from LC95s estimated for a standard susceptible population. Four field-collected populations had reduced susceptibility to cypermethrin (64-77% mortality) and to chlorpyrifos-methyl (76-77% mortality). All populations were susceptible to malathion and pirimiphos-methyl and only the DDT and pyrethroid resistant check showed resistance to all pyrethroids. Concentration-mortality curves showed non-significant levels of insecticide resistance in the field-collected populations and moderate (10- to100-fold) to very high levels (> 1000-fold) of resistance to pyrethroids in the DDT and pyrethroid resistant population. Results from insecticide bioassays with synergists (diethyl maleate, piperonyl butoxide, and triphenyl phosphate) suggested a major involvement of esterase as cypermethrin resistance mechanism, while no clear result was obtained for chlorpyrifos-methyl. Synergism results provide support for target site resistance to pyrethroids in the DDT and pyrethroid resistant population used in this study.
Species 1: Coleoptera Curculionidae Sitophilus zeamais (maize weevil)
Keywords: organophosphates, pyrethroids
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