A Z/E11-desaturase gene was cloned from the sex pheromone gland of the obliquebanded leafroller moth, Choristoneura rosaceana. The Z/E11-desaturase gene spans 1300 nt with an ORF encoding a 335 amino-acid protein, which has 81% identity to a D 11-desaturase of the redbanded leafroller moth, Argyrotaenia velutinana. A functional assay with a pYES2 yeast expression system demonstrated that the D 11-desaturase exhibits unusual substrate and stereospecificities in producing a Z11/E11 mixture (7:1) of only C14 acids. A metabolic Z9-desaturase also was cloned and expressed from fat body of this species, and produced Z9-16 >Z9-18 unsaturated fatty acids.
Species 1: Lepidoptera Tortricidae Choristoneura rosaceana (obliquebanded leafroller)
Keywords: Z/E11-desaturase gene
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