To address the needs for understanding of insecticide resistance, we have monitored genes differentially expressed (constitutive and permethrin induced) within the context of German cockroach genome response to insecticides using a combination of subtractive hybridization and cDNA array techniques. We have constructed resistant-susceptible and permethrin treated-untreated German cockroach subtractive libraries. By hybridizing cDNA arrays of PCR amplified cDNAs of 3000 randomly selected clones from each subtractive library with 32P labeled cDNA probes of resistant, susceptible, and permethrin treated German cockroaches, we obtained 2 cDNA clones from the subtractive library of resistance-susceptible German cockroaches and 1 cDNA clone from the subtractive library of permethrin treated-untreated German cockroaches that were expressed differentially. The cDNA of clone #11 from the resistant-susceptible library showed amino acid sequence homology with a cytochrome c oxidase subunit I. The cDNA of clone #33 from the resistant-susceptible German cockroach library showed amino acid sequence homology with cytochrome P450-4g15. The cDNA of clone #88 from the permethrin treated-untreated German cockroach library that was induced after treatment with permethrin showed no database match to genes of known function. The possible roles of cytochrome c oxidases and cytochrome P450s in resistance have been discussed.
Species 1: Blattaria Blattellidae Blattella germanica (German cockroach)
Keywords: pyrethroid resistance, subtractive hybridization
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