Aedes albopictus, the Asian tiger mosquito colonized the United States and Brazil early in the 1980s. Because highly polymorphic microsatellite markers have proven important population genetic tools in other mosquito vectors, they have important application in tracking this invader species. To date isolation of microsatellites from A. albopictus has been difficult. Improved methods have permitted the isolation and development of microsatellite markers for A. albopictus using 5' anchored PCR procedures. A PCR-based library enriched for microsatellites was constructed and screened for microsatellites by direct sequencing. Several microsatellites were isolated and primers were developed for each and tested for polymorphism on five populations of A. albopictus from USA, Brazil, Malaysia, Madagascar and Japan.
Species 1: Diptera Culicidae Aedes albopictus (Asian tiger mosquito)
Keywords: Population genetics, Invader species
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