Monday, 18 November 2002
D0181

This presentation is part of : Display Presentations, Subsection Ca. Biological Control

Genetic variation of Lysiphlebus testaceipes populations

Kevin A. Shufran1, Douglas B. Jones2, Albert A. Weathersbee3, Norman C. Elliott1, Keith Pike4, and Yi Chen5. (1) USDA ARS, Department of Plant Science & Water Conservation Research Laboratory, 1301 N. Western Road, Stillwater, OK, (2) Oklahoma State University, Department of Entomology and Plant Pathology, 127 NRC, OSU, Stillwater, OK, (3) USDA-ARS, US Horticultural Research Laboratory, 2001 S. Rock Rd, Fort Pierce, FL, (4) Washington State University, Entomology, 24106 N Bunn Rd, Prosser, WA, (5) Alderson-Broaddus College, Natural Science Division, Campus Box 2096, Philippi, WV

Lysiphlebus testaceipes Cresson is a solitary endoparasitoid of aphids and native to the US. It is the most widely distributed aphid parasitoid in the US and has a large host range. Furthermore, L. testaceipes is the primary parasitoid attacking cereal aphids in the Great Plains from Texas to North Dakota. In a previous study it was found that a Nebraska population had a much higher survivorship at cold temperatures than a population from Oklahoma and Texas. This suggested local adaptation to the environment and perhaps partitioning of populations. To test this, we examined a 500 bp portion of the mtDNA COI gene for sequence divergence. However, we found no variation among these populations. There was significant COI and 18S variation between the Great Plains populations and populations from Florida and Spain, suggesting partitioning by geography and/or host. Currently, we are examining the mtDNA 16S RNA gene for sequence divergence among these and other populations. Minor variation was found in the 16S region of populations from the Great Plains and the Pacific Northwest

Species 1: Hymenoptera Aphidiidae Lysiphlebus testaceipes
Species 2: Homoptera Aphididae Schizaphis graminum (greenbug)
Species 3: Homoptera Aphididae Toxoptera citricida (brown citrus aphid)
Keywords: parasitoid, biological control

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