Monday, 18 November 2002
D0180

This presentation is part of : Display Presentations, Subsection Ca. Biological Control

Specific 16s rDNA markers for detection of a hyperparasitoid species, Dendrocerus carpenteri (Hymenoptera: Megaspilidae), from an aphid parasitoid, Lysiphlebus testaceipes, and six cereal aphid species using PCR

Yi Chen, Alderson-Broaddus College, Natural Science Division, Campus Box 2096, Philippi, WV, Keith Pike, Washington State University, Entomology, 24106 N Bunn Rd, Prosser, WA, and Kevin A. Shufran, USDA ARS, Department of Plant Science & Water Conservation Research Laboratory, 1301 N. Western Road, Stillwater, OK.

16s ribosomal RNA gene sequences were amplified by the polymerase chain reaction (PCR) and sequenced from the hyperparasitoid, Dendrocerus carpenteri (Curtis), 6 strains of the primary parasitoid, Lysiphlebus testaceipes (Cresson), and 6 cereal aphid species: greenbug (Biotype E), Schizaphis graminum (Rondani); Russian wheat aphid, Diuraphis noxia (Mordvilko); bird cherry-oat aphid, Rhopalosiphum padi (L.); corn leaf aphid, R. maidis (Fitch); yellow sugarcane aphid, Sipha flava (Forbes); and English grain aphid, Sitobion avenae (F.). The PCR using primers designed on the basis of these 16s rDNA sequences, followed by agarose gel electrophoresis, successfully distinguishes D. carpenteri from the 6 strains of primary parasitoid, L. testaceipes, and all 6 cereal aphid species.

Species 1: Hymenoptera Megaspilidae Dendrocerus carpenteri
Species 2: Hymenoptera Braconidae Lysiphlebus testaceipes
Keywords: hyperparasitoid

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