Tuesday, December 11, 2001 - 4:26 PM
0661

Is 16s rRNA gene a good choice in phylogenetic studies of Hymenopteran insects?

Yi Chen, Natural Science Division, Alderson-Broaddus College, Natural Science Division, Campus Box 2096, Philippi, WV

Ribosomes are a part of the translational machinery of a cell in the insects. Ribosomal RNA (rRNA) that is encoded by rDNA is essential for cellular growth, function and survival. Consequently, the primary, secondary and tertiary structures of rRNA molecules have been conserved during evolution. Ribosomal RNAs vary in size and variable regions. The small size of 5S rRNA and extensive secondary and tertiary structures present in the 23S rRNA render them unsuitable for sequencing and further analysis. The moderate size and sequence conservation of 16S rRNA gene has made it a good candidate in phylogenetic analysis of various groups of living organisms. In this study, 16S rRNA gene is used and analyzed in comparison with other genes in the phylogenetic analysis of a highly diverse insect order, Hymenoptera.

Species 1: Hymenoptera
Keywords: 16s rRNA, phylogenetic analysis

The ESA 2001 Annual Meeting - 2001: An Entomological Odyssey of ESA