Monday, December 10, 2001 -
D0014

Low temperature scanning electron microscopy of soft-bodied arthropods

Eric F. Erbe1, Ethan Kane2, Christopher Pooley1, William P. Wergin1, and Ronald Ochoa3. (1) USDA-ARS, Nematology Laboratory, Bldg. 011A, Rm. 165, 10300 Baltimore Ave, Beltsville, MD, (2) University of Maryland, Department of Entomology, Plant Sciences Building, College Park, MD, (3) USDA-ARS, Systematic Entomology Laboratory, 10300 Baltimore Avenue, Building 005, Room 137, BARC-WEST, Beltsville, MD

Traditional scanning electron microscopy (SEM) has long been useful for imaging morphological features of hard-bodied organisms, however, this technique has not proven practical for many soft-bodied taxa because specimen preparation requires chemical fixation, dehydration, and critical point drying. Recently, low-temperature field emission SEM has been used to obtain high resolution images of a wide range of soft-bodied arthropod taxa, including mites, spiders, collembola, myriapods, as well as a diverse array of both larval and adult insects. Utilizing contact-freeze immobilization techniques, the authors were able to capture and image specimens in near-natural positions, providing insights into both behavior and ecology.

Keywords: Scanning Electron Microscopy

The ESA 2001 Annual Meeting - 2001: An Entomological Odyssey of ESA