The message corresponding to the horn fly esterase cDNA, HiaE7, appears to be present at elevated concentration in a strain of diazinon-resistant horn flies (Guerrero, F.D. 2000. Insect Biochem. Molec. Biol. 30:1107-115). The cDNA encoding this esterase was inserted into the E. coli/baculovirus recombination shuttle vector pBluBacHisC2 (Invitrogen, Carlsbad CA),and was used to construct a recombinant baculovirus expressing the horn fly esterase as a recombinant fusion protein. Expression was optimized and the recombinant protein is currently being used for construction of monoclonal antibodies (mAbs). These mAbs should be useful in development of an immunoassay to determine expression levels of this esterase in wild horn flies.
Species 1: Diptera Muscidae Haematobia iritans (horn fly)
Keywords: insecticide resistance, esterase
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