The developmental expression profile and the immunolocalization of a 56 kDa neuropeptide in Drosophila melanogaster was analyzed through a Western blot and immunocytochemistry using O1C10 mAb as a specific probe. The 56 kDa neuropeptide was isolated from the brain of D. melanogaster through a combination of immunoaffinity and 2-D gel analysis. A partial amino acid sequence analysis and the functional bioassay of the neuropeptide are in progress.
Species 1: Diptera Drosophilidae Drosophila melanogaster
Keywords: neuropeptide, Western blot
The ESA 2001 Annual Meeting - 2001: An Entomological Odyssey of ESA