Tuesday, December 11, 2001 -
D0304

Partial characterization of trypsin-like protease and molecular cloning of a trypsin-like precursor cDNA in salivary glands of Lygus lineolaris

F. Zeng1, Y. Zhu2, and A. Cohen1. (1) USDA/ARS, Biological Control and Mass Rearing Research Unit, P.O. Box 5367, Mississippi State, MS, (2) USDA/ARS, Southern Insect Management Research Unit, P.O. Box 346, Stoneville, MS

Trypsin-like enzymes from the salivary gland complex of Lygus lineolaris (Palisot de Beauvois) were purified by preparative isoelectric focusing (IEF). Lima bean trypsin inhibitor and phenylmethylsulfonyl fluoride completely inhibited the protease activity in the crude salivary gland extracts as well as the IEF purified salivary gland extracts on zymogram gels. A cDNA coding for a trypsin-like protein in the salivary glands of L. lineolaris was cloned and sequenced.

Species 1: Hemiptera Miridae Lygus lineolaris (tarnished plant bug)
Keywords: inhibitor, pH optimum

The ESA 2001 Annual Meeting - 2001: An Entomological Odyssey of ESA