A house fly strain, ALHF, was collected from a poultry farm in north Alabama, USA, with an initial 260-fold level of resistance to permethrin compared to a susceptible strain. After 5 generations of selection with permethrin in the laboratory, resistance increased to 1,800-fold. Genes that are differentially expressed between the ALHF and the susceptible strain and genes that are in response to the stimulation of insecticides in house flies have been investigated using subtractive hybridization and complementary DNA (cDNA) array techniques. Five genes that are specifically expressed in ALHF strain compared to a susceptible strain and 6 genes that are specifically expressed in permethrin-treated house flies have been identified. The function(s) of these genes and their possible role(s) in response to insecticides will be discussed.
Species 1: Diptera Muscidae Musca domestica (house fly)
Keywords: house fly, resistance, gene
The ESA 2001 Annual Meeting - 2001: An Entomological Odyssey of ESA