Three serovars of Bacillus thuringiensis (aizawai, israelensis, and kurstaki) were grown on both BHI agar and BHI broth at the temperatures of 28°, 38°, and 45° for 5 days. Colonies were chosen randomly and examined for crystal production using phase contrast microscopy. Additionally, three enterotoxin producing isolates of Bacillus cereus were obtained from the American Type Culture Collection (ATCC) and grown on BHI agar and BHI broth at 28°C. These isolates were serially transferred 10 times over a 30 day period in order to determine whether crystals would appear. All of the Bt serovars produced crystalline inclusions, including those grown at 45°C. None of the Bacillus cereus isolates produced crystals.
Species 1: Bacilliacae Bacillus thuringiensis (Bt)
Keywords: crystal
The ESA 2001 Annual Meeting - 2001: An Entomological Odyssey of ESA