ESA Annual Meetings Online Program

Computational prediction of miRNA targets in three different Asian citrus psyllid (Diaphorina citri) life stages

Monday, November 12, 2012: 8:15 AM
Ballroom G, Floor Three (Knoxville Convention Center)
Juan F. Macias Velasco , Biology, University of Texas, Tyler, Tyler, TX
Wayne B. Hunter , U.S. Horticultural Research Laboratory, USDA, Agricultural Research Service, Ft. Pierce, FL
Daymon Hail , Biology, University of Texas, Tyler, Tyler, TX
Blake R. Bextine , Department of Biology, University of Texas, Tyler, Tyler, TX
MicroRNAs (miRNA) are small RNA molecules
involved in the post-transcriptional regulation
mechanism, RNA interference (RNAi). Unlike other small
RNAs such as small interfering RNA’s (siRNA), miRNAs
are encoded in genomes. For this reason it is theorized
that miRNAs play a key role in developmental
regulation. In this study the targets of 103 pea aphid
(Acyrthosiphon pisum) in transcriptomes of 3 asian
citrus psyllid (Diaphorina citri) life stages were
determined using the miRanda algorithm. The predicted
targets were then identified using BLAST2GO
annotation. No trend between life stages
is true for all miRNAs. Implying variable importance of
miRNAs. Predicted miRNA targeting is broad, and the
methods mentioned most likely will yield viable target
prediction and identification. This process may be very
useful in the field of pest-management.