Nest density of Bombus impatiens in heterogeneous landscape surrounding vegetable farms

We collected Bombus impatiens foragers from 6 pumpkin fields located in north, central and SE Pennsylvania. Using 10 microsatellite markers, we determined relatedness among workers to estimate the number of colonies foraging within the pumpkin patch and utilizing the resource-rich floral bloom. Based on this measure and foraging distance, we can estimate the nest density of B. impatiens in the landscape surrounding the field. These conclusions will provide growers information about how many of these native bee colonies contribute pollination services to their vegetable farms. This also allows for a better understanding of native bee population dynamics and nesting habitat in heterogeneous agroecosystems.