Molecular analysis of NADPH-cytochrome P450 reductase in the bed bug, Cimex lectularius (Hemiptera: Cimicidae)

Sunday, December 12, 2010: 1:53 PM
Sunrise (Town and Country Hotel and Convention Center)
Fang Zhu , Entomology, University of Kentucky, Lexington, KY
Sarah Sams , Entomology, University of Kentucky, Lexington, KY
Zhentao Sheng , Entomology, University of Kentucky, Lexington, KY
Alvaro Romero , Entomology, University of Kentucky, Lexington, KY
Kenneth Haynes , Entomology, University of Kentucky, Lexington, KY
Michael Potter , Entomology, University of Kentucky, Lexington, KY
Subba R. Palli , Entomology, University of Kentucky, Lexington, KY
Cytochrome P450s are involved in detoxifying insecticides and plant toxins and biosynthesizing endogenous compounds. Electrons needed for these processes are transferred from NADPH to cytochrome P450 by a cytochrome P450 partner enzyme, NADPH-cytochrome P450 reductase (CPR). Using PCR-strategy, we cloned the full length of CPR cDNA from the bed bug, Cimex lectularius (Hemiptera: Cimicidae). Double strand RNA (dsRNA) mediated knock-down in the expression of CPR significantly enhanced the sensitivity to deltamethrin in a deltamethrin resistant population from the Cincinnati area (CIN-1), suggesting P450-mediated metabolic detoxification is one of mechanisms responsible for the resistance observed in this population. The developmental and tissue specific expression of the bed bug CPR was also examined.