The majority of Fruit Flies (Tephritidae) regulated as quarantine pests are known to reproduce in multiple host fruit species, often in several plant families. Methods of determining host-nonhost status include evaluations of ecology, behavior and host fruit resistance. Experimental methods for evaluating resistance or other physiological/nutritional factors in regulatory entomology have not been improved in the last 20 years. Here I present a method that evaluates mortality rates in different host fruit maturity and tissues over the development of the pest. I use a survival table approach with the Mexican fruit fly, the West Indian fruit fly, and the serpentine fruit fly using grapefruit as examples.