Unique developmental respiratory patterns in the flesh fly, Sarcophaga crassipalpis: Pupal respiratory cycles
Karl H Joplin, email@example.com, Edith Seier, firstname.lastname@example.org, and Justin Peyton, email@example.com. (1) East Tennessee State University, Biological Sciences, Department of Biological Sciences, Johnson City, TN, (2) East Tennessee State University, Mathematics, Department of Mathematics, Johnson City, TN
During the transition from wandering larvae to pupation, the flesh fly, Sarcophaga crassipalpis, undergoes a complex series of developmental changes driven by pulses of ecdysone. The wandering larvae, undergoes a series of processes where the larval cuticle barrels, or rounds up becoming smooth, hardening and sclerotizing to form the puparial case. In a couple of days at 25oC, the internal pupal cuticle forms and undergoes apolysis, separating from the tanned puparial case. A direct examination of this process is difficult since it takes place behind the opaque puparial case. We have examined the CO2 respiration during this developmental change and have found a complex but reproducible pattern of respiratory cycles during this period. This suggests a novel and unique developmentally complex control of respiration that is different from passive ventilation or discontinuous gas exchange reported in other systems.
Partial support was provided by HHMI Symbiosis Grant # 52005872.
Species 1: Diptera Sarcophagidae Sarcophagacrassipalpis (flesh fly)