Molecular characterization of multi-drug resistant enterococci from stored products and stored-product insects
Lakshmikantha H Channaiah, email@example.com, Bhadriraju Subramanyam, firstname.lastname@example.org, and Ludek Zurek, email@example.com. (1) Kansas State University, Department of Grain Science and Industry, 201 Shellenberger Hall, Manhattan, KS, (2) Kansas State University, Entomology, 123 Waters Hall, Manhattan, KS
Enterococci are ubiquitous, catalase negative, gram-positive cocci. Enterococcusfaecalis and E. faecium have emerged worldwide during the last decade as one of the leading causes of nosocomial infectious diseases. Their intrinsic or acquired resistance to many antibiotics has become a major cause of concern. In the United States, the prevalence of antibiotic-resistant enterococci is rising, in part, due to the use of antibiotics as growth promoters for farm animals. There is a concern regarding the use of antibiotics in feed for livestock production and the development of resistance in strains of clinical importance, which would limit the treatment options for infections caused by antibiotic resistant enterococci. In this study several Enterococcus species obtained from livestock facilities and feed mills, were examined for their antibiotic resistance profiles. Stored products and stored-product insects were collected in sterilized polythene bags. Enterococci were isolated from stored products and stored-product insects using selective media. Isolates were identified as Enterococcusfaecalis and Enterococcuscasseliflavus using species-specific primers. Phenotypic and genotypic analysis of these isolates for antibiotic resistance and virulence factors demonstrate that stored products and stored-product insects harbor antibiotic resistant and potentially virulent enterococci, and may act as a vehicle for transmission and spread of antibiotic resistant genes to pathogens of clinical importance.
Species 1: Coleoptera Tenebrionidae Triboliumcastaneum (red flour beetle) Species 2: Coleoptera Tenebrionidae Triboliumconfusum (confused flour beetle) Species 3: Coleoptera Dermestidae Trogodermavariabile (warehouse beetle)