Characterization of resistance to Bacillus thuringiensis in cabbage looper, Trichoplusia ni
Ping Wang, email@example.com, Jianzhou Zhao, firstname.lastname@example.org, Wendy Kain, email@example.com, Anthony Shelton, firstname.lastname@example.org, Juan Ferre, Juan.Ferre@uv.es2, Ana Rodrigo, Ana.Rodrigo@uv.es2, Alida Janmaat, email@example.com, and Judith Myers, firstname.lastname@example.org. (1) Cornell University, Department of Entomology, NYSAES, 630 W. North Street, Geneva, NY, (2) Universidad de Valencia, Departamento de Genetica, Dr. Moliner 50, 46100-Burjassot, Valencia, Spain, (3) University of British Columbia, Department of Zoology, 6270 University Boulevard, Vancouver, British Columbia, Canada
The resistance of a Bacillus thuringiensis (Bt) resistant population of cabbage looper, Trichoplusia ni, collected from commercial vegetable greenhouses, was characterized. This Bt-resistant population is mainly resistant to the Bt toxin Cry1Ac, and the resistant trait to Bt (kurstaki) appears to be autosomal, polygenic and partially recessive. To understand the mechanism of Cry1Ac resistance in this field-originated Bt-resistant T. ni strain, we established a strain of T. ni highly resistant to Cry1Ac from this original population and generated isogenic lines of T. ni. The resistance to Cry1Ac was further characterized by analyses of the inheritance of the Cry1Ac-resistant trait, protease activities and profiles, and the binding of the toxin to the midgut brush border membranes.