The spread of West Nile virus (WNV) through Kentucky has prompted concern among citizens about the potential for infection by mosquitoes, and according to a preliminary survey, homeowners also consider mosquitoes in their backyard to be a nuisance and will only tolerate one to three bites (Trout and Brown, unpublished data). As a result, pest control operators have attempted to reduce mosquito populations in neighborhoods with chemical control targeted at adult daytime resting sites, and county health departments are targeting both adults and larvae. Knowledge of mosquito species composition in backyards can help pest managers more specifically target their control efforts. Although researchers have studied the diversity and seasonal abundance of mosquitoes in several counties in Kentucky, nothing has been reported for urban landscapes in central Kentucky. The objective of this study was to determined the species composition of mosquitoes found in Fayette County urban backyards, which species are biting humans during the evening hours, and which species complexes are attracted to various traps placed in urban landscapes. Homes in each of eight neighborhoods were sampled by placing one each of the following per yard: CDC trap baited with CO2, gravid trap baited with grass- and rabbit food-infused water, and egg trap (ovitrap) baited with grass-infused water. Landings on a human subject were also observed. Species complexes were compared between traps and across the season. Results of this study will be presented.