ITS2 Polymorphisms in select container breeding Aedes/Ochlerotatus species (Diptera: Culicidae)
Brian D. Byrd, firstname.lastname@example.org and Dawn M. Wesson. Tulane University, Department of Tropical Medicine, 1430 Tulane Avenue, SL-17, New Orleans, LA
In an effort to develop a molecular assay to aid in species identification, we investigated size polymorphisms of the nuclear ribosomal DNA internal transcribed spacer region (ITS2) for the following species: Aedes aegypti, Ae. albopictus, Ochlerotatus triseriatus, Oc. bahamensis, Oc. mediovittatus, Oc. altropalpus, and Oc. japonicus. A single oligonucleotide pair designed to anneal at the 5.8 and 28s regions flanking the ITS2 produced PCR products for all seven species and generated distinguishable amplicons for Ae. aegypti, Ae. albopictus, Oc. mediovittatus, and Oc. japonicus. Derived sequence data were used to design specific internal oligonucleotides to differentiate the species with similar ITS2 amplicon sizes.
Species 1: Diptera Culicidae Aedesaegypti (yellowfever mosquito) Species 2: Diptera Culicidae Aedesalbopictus (Asian tiger mosquito) Species 3: Diptera Culicidae Ochlerotatustriseriatus (eastern tree hole mosquito) Keywords: ITS2 polymorphisms, molecular identification