Structural and conformational aspects of the interaction of pyrokinin-like neuropeptides with expressed receptors
Ronald J Nachman, email@example.com, Young-Joon Kim, firstname.lastname@example.org, Howard Williams, email@example.com, Geoffrey Coast, firstname.lastname@example.org, Reinhard Predel, email@example.com, and Michael Adams, firstname.lastname@example.org. (1) Southern Plains Agricultural Research Center, U.S. Department of Agriculture, 2881 F/B Road, College Station, TX, (2) University of California, Department of Entomology, Riverside, CA, (3) Texas A&M University, Department of Chemistry, College Station, TX, (4) University of London, School of Biology, Birkbeck College, London, England, (5) Saxon Academy of Sciences, Research Group Jena, Erberstrasse 1, Jena, Germany
The interaction of several classes of pyrokinin-like neuropeptides with their respective expressed receptors is investigated from a structural and conformational perspective. The discussion concerns a broad class of insect neuropeptides that represent products of the Capa-gene. Structural and conformational aspects critical to the interaction of the pyrokinin/PBAN class of neuropeptides and another Capa-gene product, the ecdysis-triggering hormone (ETH), with respective, expressed lepidopteran receptors are described. These ligand-receptor interactions are compared with the structural and conformational requirements for diuretic activity of the CAP2b/PVK sub-class of pyrokinin-like neuropeptides, also of the broad Capa category, in a dipteran. Insect neuropeptides of the classes selected for this discussion are implicated in the regulation of critical processes in pest insects. However, they suffer from a relative lack of biostability and bioavailability that limits their effectiveness as tools for neuroendocrinologists and as pest management agents. Biostable/bioavailable mimetic analog development of insect neuropeptides will be facilitated by a through understanding of structural/conformational aspects of the interaction with their receptors.