Previous work with monoclonal antibody MAb MS17 showed the protein MS17 is less abundant in granular cells from larvae previously injected with bacteria compared to granular cells from control larvae. MS17 protein is approximately 86 kDa; immunolocalization experiments indicated it is normally present only in vesicles of granular cells (a class of hemocytes).
Here we show that MS17-containing vesicles were more abundant (SAS GENMOD p<0.001) in hemocytes treated with thermolysin after harvesting than in non-treated hemocytes. Since incubation times are short (20-60 minutes) this indicates that thermolysin inhibited release of MS17.
This work is part of on-going work on the role of zinc and zinc-proteases in the M. sexta immune response.
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