We previously categorized heat shock protein (hsp) transcription during pupal diapause in the flesh fly Sarcophaga crassipalpis, but did not define the fine details of this expression. This study addresses three questions: 1) How early does hsp expression begin in diapause? 2) What is the order of expression change? 2) Does hsp expression persist during post-diapause quiescence? A consistent and temporally ordered pattern of hsp expression was noted during diapause inception (d1 to d15 after pupariation). Diapause-like hsp expression continued after the onset of covert development potential (i.e. post-diapause), but was terminated at the onset of overt development. This indicates an important role for hsp’s beyond the period covered by diapause under field conditions. This data also helps elucidate possible functions for the different stress proteins during diapause, and further discriminates the molecular characteristics of diapause end.
Species 1: Diptera Sarcophagidae Sarcophaga crassipalpis (flesh fly)
Keywords: diapause, heat shock proteins
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