Complementary sex determination (the production of diploid males) is present in a number of Hymenopteran species, including Habrobracon hebetor Say (Hymenoptera: Braconidae). We examined complementary sex determination in H. hebetor that were cured or infected with Wolbachia at two temperature regimes. Virgin females from isofemale lines (cured and infected) were mated to recombinant males that shared sex alleles but differed in eye color markers. Their progeny were placed in environmental chambers at either 18 or 27°C to develop into adults. Diploid males were produced in both cured and Wolbachia lines as well as at both temperature regimes. Temperature sensitive stage of eggs and implications for biological control will be discussed.
Species 1: Hymenoptera Braconidae Habrobracon hebetor
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