We determined the detectability of greenbug and bird cherry-oat aphid cytochrome oxidase II in guts of green lacewing, convergent ladybeetle and seven-spot ladybeetle by PCR. Predators were held under spring or summer temperature regimes, and fragments of different sizes were amplified. Shorter fragment lengths tend to be detectable longer, and detectabilities tend to be longer under the spring regime. Nevertheless these trends are not invariant. Also, the ranks of detectabilities among the three predators vary with aphid and fragment length. DNA detectabilities in diverse predator-prey systems may need to be determined on a case by case basis.
Species 1: Homoptera Aphididae Schizaphis graminum (greenbug)
Species 2: Homoptera Aphididae Rhopalosiphum padi (bird cherry-oat aphid)
Species 3: Neuroptera Chrysopidae Chrysoperla carnea (green lacewing)
Keywords: pcr, gut analysis
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