The ecdysteroid hormones, mainly 20-hydroxyecdysone (20E), play a pivotal role in insect development by regulating the expression of a set of genes involved in molting and metamorphosis. The salivary glands from the third instar larvae of both the wildtype and the ecdysone-deficient mutant without childrenrgl (wocrgl) of Drosophila melanogasterwere incubated with or without physiological concentrations of 20E for 24 hours and subjected to both one and two-dimensional (2-D) gel electrophoresis and silver-staining. Several woc mutant specific proteins were identified and their expression could be suppressed by 20E incubation, suggesting that 20E negatively regulate the expression of the proteins. The mutant specific proteins have been isolated from 2-D gels. Identification and characterization of the 20E suppressed, mutant specific proteins are in process.
Species 1: Diptera Drosophilidae Drosophila melanogaster (pomace fly)
Keywords: salivary gland, 20-E
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